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. 2012 May 15;23(10):1860–1873. doi: 10.1091/mbc.E11-09-0746

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© 2012 Orsi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — CLEM analysis of mRFP-Atg9 in WIPI2-depleted GFP-DFCP1 cells. (A) CLEM of WIPI2-depleted starved or (B) RF starved cells. (A) Left, GFP-DFCP1/mRFP-Atg9–expressing cell. Right, boxed area in low-magnification image and two serial sections from this region. Red arrowheads indicate tubular and vacuolar Atg9-positive membranes. Black arrowhead indicates double-membrane phagophore. (B) Top left, confocal microscopy of mRFP-Atg9 and GFP-DFCP1–positive structures (boxes 1–3). These structures were then identified in low-magnification TEM and then in higher-magnification images, and sections from three different areas (1–3) are shown. Black arrowhead indicates double-membrane phagophores. Asterisk indicates autophagosome. Bars, 50 μm (A, B), 1 μm (A, middle panel).