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. 2012 May 15;23(10):1860–1873. doi: 10.1091/mbc.E11-09-0746

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© 2012 Orsi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — (A) Live-cell imaging of HEK293/GFP-DFCP1 cell transfected with mRFP-Atg9 using widefield fluorescence microscopy. (A′) Magnified frames from the boxed region. Frames are taken from Supplementary Video S1, which started after 5 min of incubation in starvation medium. Images were captured every 5 s, and every other frame is shown. White arrowhead, mRFP-Atg9 vesicle; empty arrowhead, GFP-DFCP1. Note how the two structures orbit each other without significant overlap except at +110 s, immediately after which they separate. (B) Live-cell imaging of HEK293/GFP-LC3/mRFP-Atg9 cells by widefield fluorescence microscopy. (B′) Magnified boxed regions. Images are extracted from Supplementary Video S2. Frames were taken every 5 s, and every other frame is shown. Note how the two structures orbit each other without significant overlap except at +60 s, immediately after which they separate. White arrowhead, mRFP-Atg9 vesicle.