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. 2012 May 15;23(10):1860–1873. doi: 10.1091/mbc.E11-09-0746

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© 2012 Orsi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Regulation of mAtg9 traffic by ULK1 and WIPI2. (A) Confocal microscopy of HEK293/GFP-DFCP1 cells treated with siRNAs against RISC-free (ctrl), ULK1, WIPI2, or WIPI2 and ULK1. Cells were incubated in full medium or starved for 2 h before fixation. mAtg9 was detected by indirect immunofluorescence. Arrows indicate areas of colocalization between GFP-DFCP1 and mAtg9. In WIPI2 KD virtually every DFCP1 spot colocalizes with mAtg9. (B) The efficiency of the knockdowns in A was confirmed by Western blot using antibodies against ULK1, WIPI2, and β-tubulin. One representative experiment of three is shown.