TABLE 1:

Colocalization of mAtg9 with phagophore and autophagosome markers.

	Percentage of Atg9 with phagophore and autophagosome markers
	Total Atg9	SEM	Local Atg9	SEM
Ulk1	0.52	0.37, n = 2	15.03	1.01 n = 2
DFCP1	4.00	0.92, n = 3	23.20	4.02 n = 3
WIPI2	1.73	0.16, n = 3	15.60	1.26 n = 2
Atg16	1.08	0.89, n = 3	9.77	2.48 n = 3
LC3	4.50	0.64, n = 3	18.42	2.48 n = 3
	Phagophore and autophagosome markers
	Percentage with Atg9	SEM
ULK1	19.01	5.82, n = 2
DFCP1	15.71	3.71, n = 3
WIPI2	10.44	2.73, n = 2
Atg16	22.39	6.17, n = 2
LC3	16.31	5.81, n = 3

All quantifications were performed with Imaris software, setting an appropriate threshold, which was kept constant during the whole analysis. Images for quantification were taken under the same conditions, and about five images per experiment were quantified. The values of all images from a single experiment were averaged together, and this value was used as n = 1 for statistical analysis with Excel and Prism software.

For “total Atg9,” the signal of Atg9 and the relative marker in the whole cell were compared. For “local” colocalization, at least 20 spots that were positive for the marker of interest were picked in blind per experiment. The colocalization between Atg9 and the marker was then evaluated only in a squared area of approximately twice the diameter around each spot.