FIGURE 3:

Multiple members of the annexin family interact with proAREG and ADAM17. (A) HT1080 cells were transiently transfected with ANX-V5 and proAREG expression vectors. Cells were immunoprecipitated with an anti-V5 antibody. Western blotting was carried out using anti–AREG-N antibody. (B) AP activity was measured in the conditioned media from HT1080 cells stably expressing AP-tagged proAREG. Cells treated with siRNA were stimulated with 20 nM TPA for 30 min. Data represent the mean ± SEM; **p < 0.01; n.s., not significant. (C) AP activity was measured as in (B). Data represent the mean ± SEM; *p < 0.05; **p < 0.01. (D) HT1080 cells were transiently transfected with ANX-V5 and ADAM17 expression vectors. Cells were immunoprecipitated with an anti-V5 antibody. Western blotting was carried out using an anti-ADAM17 antibody.