FIGURE 7:

Annexins are involved in UVB-induced EGFR transactivation via ADAM-mediated proAREG shedding in keratinocytes. Keratinocytes were transfected with siRNA. The day before the UVB experiment, cells were incubated in BHE-free medium. After 30 min of UVB irradiation, cells were immunoprecipitated with an anti-EGFR antibody. Cell lysates and precipitates were analyzed by Western blotting. The intensity of UVB-induced phosphorylated EGFR and JNK was normalized to the respective total protein content and represents fold-change relative to UVB-untreated cells. Soluble peptides present in the conditioned medium were extracted with a 20% TCA solution. Released AREG in the conditioned medium was detected by anti–AREG-N antibodies.