Skip to main content
. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Structure. 2012 Apr 12;20(5):814–825. doi: 10.1016/j.str.2012.03.007

Figure 4. Cross-validation of crystal structure and crosslink data for yTRiC.

Figure 4

(A) Distance distribution for the closed state yTRiC heterotypic crosslink dataset. The median heterotypic Cα-Cα crosslink distance in the model is 16.4 Å. (B) Heterotypic crosslink Cα pair distances for inter ring and intra ring subunit pairings observed in the refined XL-MS-based crystal structure. The crosslinks compatible with the XL-MS arrangement are highlighted in blue; crosslinks mapping to the grey box exceed the cutoff. (C) Alignment showing unique insertions in yTRiC subunits CCT6 and CCT4. (D) Unbiased 2Fo-Fc electron density for these insertions at 1 σ. The thermosome structure is shown in black for comparison. See also Figure S4 and Table S4.