Fig. 2.

Real-time PCR after 4 and 24 h of differentiation with 500 μM GTP in SM in C2C12 cells. Ppp3ca, Gsk3b, Pax7, and Cox7a1 mRNA expression levels in C2C12 cells differentiated with SM for 4 and 24 h in the presence of 500 μM GTP and GTPγS (inset). Data are relative quantities of transcripts for these target genes, each versus beta-actin gene expression. At 24 h of differentiation in SM in the presence of 500 μM GTP, the relative levels of Ppp3ca, Gsk3b, Pax7, and Cox7a1 mRNAs were significantly increased. Each value represents the relative quantification of each gene at each time point. Insert: Pax7 mRNA expression levels comparing GTP (as 100% at 4 and 24 h) and GTPγS, with similar levels of expression seen. Data are representative of one of three independent experiments