Fig. 6.

CCL2 causes lysosomal exocytosis in microglial cells. a Immunofluorescence for P2X4R (green) and LGP85 (red), a marker of lysosome, in primary cultured microglial cells treated with or without CCL2 (100 ng/mL) for 30 min. b The release of the lysosomal enzyme β-hexosaminidase from microglial cells was assayed by measuring its activity in the supernatants of microglial cultures collected at 30 min after CCL2 (100 ng/mL) or ionomycin (5 μM) stimulation. The experiments were repeated six times and the data are means ± SEM. *P < 0.05, **P < 0.01 compared with unstimulated control microglia. Scale bar: 10 μm