Fig. 4.
Influence of cholesterol depletion of intrinsic human P2X4 receptor properties. a Concentration–response relationships for ATP in control cells (squares), methyl-β-cyclodextrin (triangles) or filipin III (circles) treated cells (N = 10 cells each). Whole-cell currents in perforated patch configuration normalised to maximum response. b Rundown of peak current with repeated ATP application in control (squares), methyl-β-cyclodextrin (triangles) or filipin III (circles) treated cells (N = 8 cells each). Peak currents are normalised to peak after first application. c Representative current traces showing current decay in control and fillipin III-treated cells. Currents are normalised to peak response to 100 μM ATP. d Pooled average demonstrating current decay during agonist occupancy is independent of membrane cholesterol (N = 8 cells each)