Fig. 5.

NTPDase2 overexpression-induced alterations in platelet reactivity. A The ATPase, ADPase, and AMPase activities in platelets from control (animals not submitted to surgery), C6EYFP or C6NT2 glioma-implanted rats. B Platelet aggregation from C6EYFP or C6NT2 glioma-implanted rats. The platelet aggregation results are expressed as the percentage in relation to control (animals not submitted to surgery). C Platelet count from C6EYFP or C6NT2 glioma-implanted rats; C6EYFP was taken as control (platelet reference value for Wistar rats 700 × 10³ μL). D The presence of platelet activation and recruitment to lung tissue was assessed by immunohistochemical analysis of P-selectin positive cells, a platelet activation marker. Arrows indicate P-selectin-positive platelets (magnification ×40). The values represent the mean ± SD of at least three independent experiments performed in triplicate. Data were analyzed by ANOVA followed by post-hoc comparisons (Tukey–Kramer test). *P < 0.05 and ***P < 0.001, significantly different from controls