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A: Cos-7 cells were transfected with expression vectors for FLAG-Runx2 and HA-G9a (500 ng each in 6-well dishes). Cell extracts were immunoprecipitated with FLAG antibodies, and the immunoprecipitates were analyzed by immunoblotting using antibodies against HA epitope. B: GST or GST-G9a on glutathione agarose beads was incubated with in vitro transcribed and translated 35S-labeled Runx2. The bound protein fraction was analyzed by SDS-PAGE and autoradiography.
