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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Cell Biochem. 2012 Jul;113(7):2406–2414. doi: 10.1002/jcb.24114

Fig. 4. Enhancement of Runx2-mediated transcription by G9a in a transient reporter assay.

Fig. 4

CV1 cells in 12-well plates were transfected with the 6XOSE2-Luciferase reporter plasmid illustrated in A (200 ng/well) alone or together with expression vectors for Runx2 (1 ng) and either HA-tagged G9a full length (G9a) or HA-G9a(H/K) methyltransferase-deficient mutant (H/K) (50, 100 and 200 ng). After transfection the cells were grown for 48 h before they were subjected to luciferase assays (B) and immunoblot analysis using antibodies against HA and actin (C).

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