Fig. 5. Runx2 recruits G9a to the regulatory elements of its target genes.

C4-2B/Rx2^dox cells were plated in 15-cm dishes and cultured in media supplemented with CSS for two days and then treated with dox or vehicle for an additional 16 h before ChIP analysis using antibodies against FLAG to detect Runx2 (A) or against G9a (B). Immunoprecipitated DNA was analyzed with primers (Table 1) designed to amplify the Runx2-occupied regions of the indicated target genes.