Table 1.

Sequences for the primers and oligonucleotides

Gene	Forward (5′ to 3′)	Reverse (5′ to 3′)
CSF-2	ATGTGAATGCCATCCAGGAG	AGGGCAGTGCTGCTTGTAGT
CST7	TCCCAGGACCTTAACTCACG	GCTTCAAGGTGTGGTTGGTT
CXCL12/SDF-1	ATGAACGCCAAGGTCGTG	CTTTAGCTTCGGGTCAATGC
GAPDH	GTCATGGGTGTGAACCATGAGA	GGTCATGAGTCCTTCCACGATAC
MMP9	TTGACAGCGACAAGAAGTGG	GCCATTCACGTCGTCCTTAT
OC	GGCAGCGAGGTAGTGAAGAG	CTGGAGAGGAGCAGAACTGG
PGC	ACAGGCACCTCTCTGCTAACT	AGTAGCCGTTGTTACTGAGGAT
PIP	GTACGTCCAAATGACGAAGTCAC	CAGCAGCATCATCAGGGCAGATG
RASD1	GTGTTCAGTCTGGACAACCGC	CTGCTCGATCTCGCGCTGGTC
RunX2	CACGAATGCACTATCCAGCCAC	CGCCAAACAGATTCATCCATTC
Primers for qPCR in ChIP
CSF-2	GAAGCTTGGCTGAATAGATGC	ACACCAGACATATGAAGCAACATC
PGC	TCTCTCTTATCGCTTGCACCTCCT	TAGTCTAATCGCTGCCTCCCTGC
Primers for shRNA expression
shG9a_5 Sequence targeting nucl. 866–888 (286–294 amino acid residues)
Sense oligo: 5′-CTTGTGGAAAGGACGAAACACCGACAGCAAGTATGAAGTTAAA GCtcTTCAAGAGAGAGCTTCAACTTCAGACTTGCTGTCTTTTTCTGCAGTTTT
Reverse oligo: 5′-3′ AAAACTGCAGAAAAAGACAGCAAGTCTGAAGTTGA AGCTCTCTCTTGAAGAGCTTTAACTTCATACTTGCTGTCGGTGTTTCGTCCTTTCCACAAG
shG9a_7 Sequence targeting nucl. 280–302 (91–99 amino acid residues)
Sense oligo: 5′-CTTGTGGAAAGGACGAAACACCGGATGAATCTAAGAATCTGGA GGgaTTCAAGAGATCCCTCAAGATTCTCAGATTCATCCTTTTTCTGCAGTTTT
Reverse oligo: 5′-AAAACTGCAGAAAAAGGATGAATCTGAGAATCTTGAGGGATCTCTTGAAT CCCTCCAGATTCTTAGATTCATCCGGTGTTTCGTCCTTTCCACAAG
shNS Nonspecific sequence
Sense oligo: 5′-CTTGTGGAAAGGACGAAACACCGGGTAGGTTCAACTAGCAAGAC TCTTTCAAGAGAAGAGTCCTGCTAGTCGAACCTACCCTTTTTCTGCAGTTTT
Reverse oligo: 5′-AAAACTGCAGAAAAAGGGTAGGTTCGACTAGCAGGACTCTTCTC TTGAAAGAGTCTTGCTAGTTGAACCTACCCGGTGTTTCGTCCTTTCCACAAG
siG9a sequences from Dharmacon SMART Pool
siRNA 5	GGACCUUCAUCUGCGAGUA
siRNA 6	GAACAUCGAUCGCAACAUC
siRNA 7	GGAGGUAGCCCGUUACAUG
siRNA 8	GGAGAGGUGUACUGCAUAG