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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Struct Biol. 2012 Jan 10;178(2):165–176. doi: 10.1016/j.jsb.2012.01.004

FIGURE 6.

FIGURE 6

Improvement in separation of conformations with bundling of spikes. Contour plots of the 2D histograms for the scatter-clouds generated after iterative classification and alignment on mixtures, where the sub-tomograms from individual spikes were averaged together prior to alignment and classification to include 1 (control), 2, 5, 10, 20 or 30 spikes. The improvement in separation is observed both for mixtures of SIVmac239 and SIV CP-MAC data sets (see Figure 3b for comparison) and for the mixtures of SIVmac239 and SIV CP-MAC+sCD4+7D3 mixtures (see Figure 5b for comparison). The numbers beneath the panels indicate the average number of sub-volumes included in each “bundle”.