Fig. 2.

Effects of aldosterone with losartan on renal sodium transporters. Immunoblots showing the effects of aldosterone with losartan on the NCC (a), the ENaC and the water channel AQP2 (b). In addition, the effects of aldosterone with losartan on two regulatory kinases, WNK4 and SPAK, are shown (c). Whole kidney homogenates were differentially centrifuged to obtain plasma membrane fractions (used for all transport proteins) and intracellular fractions (used for the regulatory kinases). Densitometry was normalized for actin. *P < 0.05 by analysis of variance and post hoc test