Fig. 4.

Male mice are reported to display a similar pattern of oestrogen receptor expression as females, but a constantly low level of oestrogen expression. Therefore, we also analysed anxiety-related behaviour in male mice 2 h after treatment with 0.025 mg β-oestradiol/kg or 1 mg/kg G-1 (G-1 2 h). In addition, we included one group of animals that were treated with G-1 and b-oestradiol 2h before testing (Oest + G1). Their behaviour was analysed in the elevated plus maze (a), the light–dark choice (b), and the open field tests (c). Numbers in the columns indicate the number of animals in the respective groups. *P < 0.05, **P < 0.01 vs. DMSO