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Most but not all SC cells relaxed dose-dependently in response to (a) Y-27632, (b) DBcAMP, and (c) isoproterenol. Cells were incubated with individual drugs at multiple doses for 30–0 mins before stiffness measurement. A G-to-G0 ratio smaller than 1 indicates cell softening; G is cell stiffness with drug treatment and G0 is cell stiffness with vehicle treatment. Each data point is median value of over 1000 beads at passage 2; the error bars are standard errors.
