Figure 6.

Isoproterenol decreases the traction forces of SC cells from most but not all donors, consistent with its donor-dependent effects on cell stiffness. (a) Single SC cells exert traction when plated on a deformable substrate. Left: phase-contrast image; middle and right: traction intensity map before and 10 min after 10 µM isoproterenol (iso) treatment, respectively. While the SC58 cell diminished its traction in responses to isoproterenol (top), SC61 did not (bottom). (b) r.m.s. traction before and 10 min after 10 µM isoproterenol treatment for the five SC strains. Seven to 11 cells were tested for each donor. Error bars in (b) are standard errors, and statistical significance of the traction decrease is indicated as *p < 0.05, **p < 0.01, n.s. as p > 0.05 or p-value itself. Violet bars, baseline; purple, isoproterenol.