Figure 3. Kif3a^cko cortical progenitors cycle faster with a reduction in G1.

(A and B) Representative immunostaining of E12.5 Labeling Index analysis. Ki67 in green and BrdU in blue. (C) Kif3a^cko progenitors cycle faster than Kif3a^het progenitors shown by quantification of E12.5 labeling index. (D and E) Representative flow cytometry traces of progenitors sorted based on DNA content using propidium iodide and fit by ModfitLT. (F) Graph of ModfitLT values showing G1 is shorter in Kif3a^cko progenitors with a corresponding increase in S phase. (G and H) E14.5 Kif3a^cko embryos administered a BrdU pulse 24-hours earlier show three bands or BrdU + cells whereas Kif3a^het embryos only show two.