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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Dev Neurobiol. 2012 Jun 21;72(9):1196–1212. doi: 10.1002/dneu.20985

Figure 4. Decreased processing of Gli3 and upregulation of Shh target genes in Kif3acko mice.

Figure 4

(A) Western blot analysis of Gli3FL/Gli3Rep and CyclinD1 normalized to PCNA levels show an increase in Gli3FL/Gli3Rep ratio and Shh target genes (Ptc and Cyclin D1) in Kif3acko cortices. There is no change in phospho-ERK, β-catenin, or NotchAct expression. The PCNA loading control was used to correct for the number of progenitors. (B–E) In situ hybridization shows many Shh-target genes are upregulated in Kif3acko cortices including CyclinD1 (C), Ptc (D), and Fgf15 (E) while Gli1 (B) is not.