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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Dev Neurobiol. 2012 Jun 21;72(9):1196–1212. doi: 10.1002/dneu.20985

Figure 5. Increased Gli3FL/Gli3Rep ratio is sufficient to phenocopy cilia mutant.

Figure 5

(A and B) Graphs of labeling indices calculated for (A) Gli3+/xt, Gli3+/Δ68 and Gli3Δ68/xt and (B) Nestin Cre +; Gli3fl/+ and Nestin Cre +; Gli3fl/xt of E12.5 embryos showing an increased cycling rate for Gli3Δ68/xt and Nestin Cre +; Gli3fl/xt cortical progenitors. (C) Western blot analysis of E14.5 cortical caps showing an increased Gli3FL/Gli3Rep in Gli3Δ68/xt mice with a corresponding increase in Ptc and CyclinD1. Quantitation of Gli3FL/Gli3Rep normalized to PCNA levels above. (D–G) In situ hybridization for Shh target genes showing an increase in Cyclin D1 (E), Ptc (F), and Fgf15 (G) with no increase in Gli1 (D) expression.