Induction of apoptosis in SCC cell lines ME-180PT, and UMSCC-5 treated with G2535 (25 μM), cisplatin (Cis 1 μM) and the combination (A) B-DIM (25 μM), cisplatin (Cis 1 μM), and the combination (B) was evaluated by the ELISA assay. There was a significant induction of apoptosis observed in both the cell lines, but the amount of induction in ME-180PT was much higher than UMSCC-5 cell line treated with both the combinations as compared to cells treated with either agent alone.