Fig. 6.
DNA binding activity of NF-κB DNA in nuclear protein extracts of ME-180PT and UMSCC-5 cells as assessed by electrophoretic mobility shift assay (EMSA). Control, Cis (1 μM), B-DIM (25 μM), G2535 (25 μM), Cis + B-DIM, Cis + G2535. Significantly greater down-regulation of NF-κB DNA binding activity was observed in cells treated with the combination compared with either agent alone in ME-180PT or UMSCC-5 cell lines. Top panel represents NF-κB DNA binding activity with the densitometric quantification is shown in the bottom panel.