Figure 6.
Sequence substitutions in the PMF map of an extended ssDNA molecule. (A) Atomic structures of three-nucleotide DNA molecules with similar conformations but different sequences. (B) The difference in the K+ PMF maps generated from the structures shown in panel A. A cross section of the map is shown with the position of the substituted nucleotide shown for reference. (C) The difference in the K+ PMF maps of isolated nucleotides transformed so that the bases match the structures in panel A. The changes in the PMF map upon the substitution from an A base to a T base are similar between the three-nucleotide DNA molecule and the isolated nucleotides, although there are some obvious differences.