Figure 5.

The TrxG Protein Ash1L Is Recruited to the FSHD Locus and De-Represses 4q35 Genes

(A) ChIP for Ash1L, Chd7, Mll1, and IgG on chr4/CHO cells in the repressed (control) or de-repressed (AZA+TSA) states. ChIP was analyzed by qPCR with primers for NDE and DBE and expressed as percentage of input. The error bars represent SEM.

(B) ChIP for ASH1L in control and FSHD primary muscle cells. ChIP was analyzed by qPCR with primers for NDE and expressed as percentage of input normalized to IgG. The error bars represent SEM.

(C–F) Chr4/CHO cells stably expressing control shRNA or shAsh1L. Knockdown after AZA+TSA treatment evaluated by qRT-PCR (C), immunoblotting (D), and ChIP on NDE (E). Expression of 4q35 genes and Gapdh, as control, was analyzed by qRT-PCR (F). Results are expressed over β-actin. The error bars represent SEM.