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. 2012 May 11;149(4):819–831. doi: 10.1016/j.cell.2012.03.035

Figure S8.

Figure S8

Ash1L Dimethylates Lysine 36 on Histone H3 at the FSHD Locus, Related to Figure 6

(A) ChIP-qPCR for enrichment on total H3 of H3K4me3, H3K36me2 and IgG, as control, performed on human chr4/CHO cells in the repressed (control) or in the de-repressed (AZA+TSA) state. ChIP material was analyzed by qPCR with primers specific for the NDE region. The error bars represent SEM.

(B) ChIP-qPCR for enrichment on total H3 of H3K4me3, H3K36me2 and IgG, as control, performed in control shRNA or shAsh1L expressing cells in the de-repressed state (AZA+TSA). ChIP material was analyzed by qPCR with primers specific for the NDE region. The error bars represent SEM.

(C) ChIP-qPCR for enrichment on total H3 of H3K36me2 and IgG, as control, in control shRNA or shDBE-T expressing cells in the de-repressed state (AZA+TSA). ChIP material was analyzed by qPCR with primers specific for the NDE region. The error bars represent SEM.

(D) ChIP for Ash1L and IgG, as control, on human chr4/CHO cells in the repressed (control) or de-repressed (AZA+TSA) state. Analysis was performed by qPCR with primers specific for ANT1, FRG1, FRG2 promoters and NDE. Results are expressed as relative percentage of input. The error bars represent SEM.

(E) ChIP-qPCR for enrichment on total H3 of H3K36me2 and IgG, as control, performed on human chr4/CHO cells in the repressed (control) or in the de-repressed (AZA+TSA) state. Analysis was performed by qPCR with primers specific for ANT1, FRG1, FRG2 promoters and NDE. Results are expressed as relative percentage of H3. The error bars represent SEM.