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. Author manuscript; available in PMC: 2012 May 14.
Published in final edited form as: Nat Struct Mol Biol. 2009 Mar 8;16(4):449–451. doi: 10.1038/nsmb.1569

Figure 1. H3R2me1 does not block Set1 complex activity towards H3K4.

Figure 1

(a) Pull-down assays using synthesized peptides and recombinant GST-Spp1PHD or GST-only as a negative control. Equal loading of peptides is monitored by coomassie staining. (b) Chromatin immunoprecipitation (ChIP) analysis of logarithmically growing yeast cells using antibodies towards Myc-Spp1, H3R2me1 and H3R2m2a. Error bars represent s.e.m for duplicate experiments. (c) In vitro methyltransferase assays using purified Set1 complex and synthesized peptides. Equal amounts of peptides were used in the methyltransferase reactions as shown by coomassie staining.