Figure 2.

Knockdown of HPK1 in 1,25D-sensitive HL60 and U937 cells decreases 1,25D-induced differentiation, downstream signaling and differentiation-related TFs. Cells were pre-treated with siRNA-HPK1 (siHPK1) or scrambled-siRNA (siRNA) for 24 h, then 10 nM 1,25D was added for another 48 h. Differentiation markers CD11b and CD14 were detected by FC, mean ± SD values are shown, n = 3. Representative images of CD11b (x axis) and CD14 (y axis) determinations are shown in the left side of each figure. (A) HL60 cells; (B) U937 cells. (C) Demonstration of HPK1 decrease, activation of several components of MEKK1-JNK-AP1 signaling cascade and expression of differentiation-related TFs as detected by western blots. Calregulin is a loading control.