Figure 2.

Nutlin-3 inhibits proliferation and induces apoptosis of KS tumor cells. (A) Growth curves of HUVEC, KS-SLK1 and TIVE-KSHV cells cultured in the presence of Nutlin-3 (5 nM) or placebo (DMSO). Identical amounts (5 × 10⁴) of cells were initially seeded in 1 ml media, which was changed daily with fresh Nutlin-3 or DMSO. Results represent the mean values from three replicates; (B) DAPI nuclear staining of TIVE-KSHV cells after a 4-d treatment with Nutlin-3 and DMSO, respectively; (C) percentages (%) of apoptotic HUVEC, TIVE-KSHV and KS-SLK1 cells detected by DAPI staining (from B); (D) agarose (0.7%) gel electrophoresis analysis of total genomic DNA purified from HUVEC, TIVE-KSHV and KS-SLK1 cells that were treated with Nutlin-3 and DMSO, respectively, for 3 d.