Skip to main content
. 2012 Apr 1;11(7):1393–1399. doi: 10.4161/cc.19756

Figure 4.

Figure 4

Nutlin-3 inhibits expression of Ang-2. (A) Agarose (1.2%) gel electrophoresis analysis of real-time RT-PCR products of Ang-2 and actin transcripts from KS-SLK1 and TIVE-KSHV cells at 48 hpt with Nutlin-3 and DMSO; (B) relative levels of Ang-2 mRNA from KS-SLK1 and TIVE-KSHV cells at 48 hpt with Nutlin-3 and DMSO; (C) western blot detection of Ang-2 protein from KS-SLK1 and TIVE-KSHV cells at 48 hpt with Nutlin-3 and DMSO. The level of tubulin was used for calibration of sample loading; (D) average serum levels of Ang-2 from five Nutlin-3-treated and five placebo-treated mice as determined by ELISA.