Figure 7.

Visualizing the “reverse Warburg effect” by double labeling with MCT4 and TOMM20: Lymph node metastasis. Paraffin-embedded sections of human breast cancer-positive lymph nodes were immunostained with antibodies directed against MCT4 (red color) and TOMM20 (brown color). Slides were then counterstained with hematoxylin (blue color). Note that MCT4 staining is predominantly localized to the cancer-associated lymph node stroma. In contrast, TOMM20 is strongly associated with the metastatic breast cancer cells. Original magnification, 60x.