Fig. 7.

Identification of an S-box in the ACBP3 5'-flanking region. (A) Nucleotide sequences of double-stranded oligonucleotides used in EMSAs. The mutated nucleotides in S-box-(–516/–512)-mut and their corresponding sequences in S-box-(–516/–512)-wt are shown in bold. (B) Interaction of nuclear extracts from 5-week-old Arabidopsis leaves with S-box-(–516/–512)-wt and S-box-(–516/–512)-mut probes. Crude nuclear extracts (4 μg) from 48 h pathogen-infected (Pseudomonas syringae pv. tomato DC3000, lanes 2, 4, 6, and 9) or MgCl₂-treated (control) Arabidopsis (MgCl₂, lanes 3, 5, 7, and 10) were incubated with biotin-labelled S-box-(–516/–512)-wt (lanes 2–7) or S-box-(–516/–512)-mut (lanes 9 and 10) in the absence (lanes 2, 3, 9, and 10) or presence of a 50-fold molar excess of unlabelled competitor, S-box-(–516/–512)-wt (lanes 4 and 5), or S-box-(–516/–512)-mut (lanes 6 and 7). Lanes 1 and 8 are free probes without addition of crude nuclear extracts.