Table 1.
List of the differentially H2O2-responsive proteins in TBY-2 cells
| Spot | Protein name and plant species | NCBI accession number | EST NCBI accession number | Fold variation |
Statistical significancea |
Mascot score/peptide number | Mol. wt (kDa)/pI |
|||
| 30 min | 3 h | 30 min | 3 h | Theoretical | Expected | |||||
| Redox homeostasis | ||||||||||
| 1 | Thioredoxin peroxidase, Nicotiana tabacum | gi|21912927 | –6 | <0.01 | 0.0001 | – | 225/5 | 30.0/8.2 | 24.0/5.0 | |
| 2 | Thioredoxin peroxidase, Nicotiana tabacum | gi|21912927 | –2.4 | –1.7 | 0.005 | 0.007 | 187/5 | 30.0/8.2 | 23.0/4.7 | |
| 3 | Thioredoxin peroxidase, Nicotiana tabacum | gi|21912927 | 1 | –1.9 | – | 0.01 | 314/7 | 30.0/8.2 | 32.0/6.6 | |
| 4 | Peroxiredoxin-2E, chloroplastic, Arabidopsis thaliana | gi|143360522 | –1.5 | –1.7 | 0.05 | 0.04 | 101/2 | 22.5/7.6 | 17.0/5.1 | |
| 5 | Peroxiredoxin, putativeb, Ricinus communis | gi|255575353 | gi|225317455 | <0.01 | 1 | – | – | 189/5 | 23.7/7.6 | 16.5/5.4 |
| 6 | Peroxiredoxinb, Ipomoea batatas | gi|37783267 | gi|52834488 | 3.9 | 8 | 0.002 | 0.003 | 275/6 | 20.7/8.8 | 20.0/5.9 |
| 7 | Thioredoxin H-type 1, Nicotiana tabacum | gi|267124 | –1.9 | 1 | 0.030 | – | 178/4 | 14.1/5.6 | 13.7/5.5 | |
| 8 | Disulphide oxidoreductase, putativeb, Ricinus communis | gi|255575237 | gi|761563 | –1.6 | 1 | 0.04 | – | 321/6 | 39.3/8.2 | 35.0/5.6 |
| 9 | Ascorbate peroxidase, Nicotiana tabacum | gi|559005 | 2.3 | 3.6 | 0.03 | 0.03 | 125/3 | 27.3/5.4 | 27.0/5.4 | |
| 10 | Ascorbate peroxidase, Nicotiana tabacum | gi|559005 | 1 | –1.5 | – | 0.05 | 507/11 | 27.3/5.4 | 27.0/5.7 | |
| 11 | Ascorbate peroxidase, Nicotiana tabacum | gi|76869309 | 1 | –4 | – | 0.03 | 441/9 | 27.0/5.4 | 27.0/6.1 | |
| 12 | Thylakoid-bound ascorbate peroxidase, Nicotiana tabacum | gi|4996604 | –2.4 | –4 | 0.03 | 0.02 | 891/15 | 42.0/7.6 | 32.0/5.7 | |
| 13 | Thylakoid-bound ascorbate peroxidase, Nicotiana tabacum | gi|4996604 | –1.7 | –2.4 | 0.03 | 0.04 | 150/5 | 42.0/7.6 | 32.0/6.0 | |
| 14 | Superoxide dismutase [Fe]chloroplastic, Nicotiana plumbaginifolia | gi|134642 | <0.01 | <0.01 | – | – | 300/6 | 23.0/5.5 | 23.0/5.7 | |
| 15 | Probable glutathione S-transferaseb, Capsicum annuum | gi|60459397 | gi|76871463 | 4.7 | 6 | 0.01 | 0.002 | 135/4 | 25.4/5.5 | 26.0/5.9 |
| 16 | Glutathione S-transferaseb, Solanum commersonii | gi|148616162 | gi|83420783 | –1.9 | 1 | 0.03 | – | 202/5 | 23.8/5.9 | 25.0/6.8 |
| 17 | Glyoxalase Ia, Solanum lycopersicum | gi|2494844 | gi|190145651 | –1.6 | 1 | 0.02 | – | 268/6 | 20.7/5.3 | 21.0/4.9 |
| 18 | Flavodoxin-like quinone reductase 1b, Arabidopsis thaliana | gi|15239652 | gi|83420109 | –1.8 | 1 | 0.006 | – | 357/7 | 21.7/5.9 | 25.0/6.7 |
| Cell rescue | ||||||||||
| 19 | Chilling-responsive protein, Nicotiana tabacum | gi|153793260 | –2 | 2.3 | 0.05 | 0.03 | 114/4 | 36.0/4.9 | 39.0/4.9 | |
| Protein synthesis | ||||||||||
| 20 | Eukaryotic translation initiation factor 3 subunit, putativeb, Ricinus communis | gi|255550315 | gi|76867840 | –1.8 | 1.7 | 0.05 | 0.03 | 459/10 | 26.9/5.8 | 25.6/5.6 |
| 21 | Eukaryotic initiation factor 4A-11, Nicotiana tabacum | gi|2500518 | 1.6 | 3.2 | 0.05 | 0.007 | 735/17 | 47.2/5.4 | 47.0/5.3 | |
| 22 | Eukaryotic initiation factor 4A-9b, Nicotiana tabacum | gi|2500517 | gi|190874337 | 1 | –1.8 | – | 0.001 | 580/11 | 47.0/5.5 | 45.0/5.4 |
| 23 | Eukaryotic initiation factor 4A-9, Nicotiana tabacum | gi|2500517 | 1.7 | 2.5 | 0.04 | 0.008 | 691/15 | 47.0/5.5 | 46.0/5.3 | |
| 24 | Eukaryotic initiation factor 4A-15, Nicotiana tabacum | gi|2500521 | 1 | 2 | – | 0.008 | 1214/23 | 46.9/5.4 | 45.0//5.4 | |
| 25 | Eukaryotic translation initiation factor 5A-2c, Nicotiana plumbaginifolia | gi|124226 | 1 | <0.01 | – | – | 60/1 | 17.6/5.6 | 17.3/5.5 | |
| 26 | Eukaryotic translation elongation factor, putativeb, Ricinus communis | gi|255544686 | gi|92027234 | 2 | 1 | 0.001 | – | 441/15 | 94.0/5.9 | 105.0/6.3 |
| 27 | Elongation factor 1-beta/EF-1-betav, Arabidopsis thaliana | gi|145324076 | gi|76867651 | 1 | –1.7 | – | 0.006 | 493/11 | 28.7/4.6 | 31.4/4.5 |
| 28 | Ribosomal protein L2-like, Solanum tuberosum | gi|81074776 | 7.3 | 1 | 0.03 | – | 100/3 | 28.7/10.6 | 31.0/6.7 | |
| 29 | Glycyl-tRNA synthetase, putativeb, Ricinus communis | gi|255543218 | gi|190806778 | 2.6 | 1 | 0.07 | – | 223/6 | 77.3/6.6 | 66.5/6.2 |
| 30 | Glycyl-tRNA synthetase, putativea, Ricinus communis | gi|255543218 | gi|190878443 | <0.01 | 1 | – | – | 75/2 | 77.3/6.6 | 69.0/6.3 |
| Chaperones | ||||||||||
| 31 | Mitochondrial small heat shock proteinc, Solanum lycopersicum | gi|3492854 | gi|92010828 | 1 | 1.9 | – | 0.04 | 78/1 | 23.8/6.5 | 21.3/4.8 |
| 32 | Heat shock protein, putative, Ricinus communis | gi|255581792 | 1 | –1.5 | – | 0.04 | 498/8 | 90.0/5.2 | 92.0/5.1 | |
| 33 | Heat shock protein, Solanum lycopersicum | gi|68989120 | <0.01 | –2.9 | – | 0.009 | 276/6 | 110/6.2 | 102.0/6.0 | |
| 34 | Hsp70-binding protein, putative, Ricinus communis | gi|255581500 | gi|224704536 | 1 | –1.6 | – | 0.009 | 169/4 | 39.2/5.2 | 41.4/5.0 |
| 35 | Heat shock 70 kDa protein, putativeb, Ricinus communis | gi|255574576 | gi|92037393 | 1 | –1.6 | – | 0.009 | 227/4 | 94.0/5.2 | 110.0/5.6 |
| 36 | Heat shock 70 kDa protein, putativeb, Ricinus communis | gi|255574576 | gi|92027886 | 1 | –1.8 | – | 0.02 | 262/2 | 94.0/5.2 | 110.0/5.5 |
| 37 | Molecular chaperone Hsp90-1, Nicotiana benthamiana | gi|38154482 | 1 | –2.2 | – | 0.02 | 404/9 | 80.0/4.9 | 83.0/4.9 | |
| 38 | Heat shock protein, putative, Ricinus communis | gi|255554571 | –5.2 | 1 | 0.03 | – | 527/10 | 71.0/6.1 | 60.2/5.3 | |
| 39 | Hypothetical protein cpn60, Vitis vinifera | gi|225442531 | –2 | –1.7 | 0.02 | 0.04 | 589/11 | 65.3/5.6 | 60.2/5.3 | |
| 40 | Putative luminal binding proteina, Corylus avellana | gi|10944737 | gi|190753562 | <0.01 | –2 | – | 0.02 | 618/23 | 73.0/4.9 | 74.0/5.1 |
| 41 | Chaperonin containing t-complex protein 1, epsilon subunit, tcpe, putativeb, Ricinus communis | gi|255547962 | gi|92040148 | 2.3 | 1.6 | 0.03 | 0.04 | 488/11 | 59.2/5.5 | 60.0/5.6 |
| 42 | Chaperonin containing t-complex protein 1, epsilon subunit, tcpe, putativeb, Ricinus communis | gi|255547962 | gi|92040148 | <0.01 | <0.01 | – | – | 173/4 | 59.2/5.5 | 55.4/5.2 |
| 43 | Chaperonin containing t-omplex protein 1, gamma subunit, tcpe, putativeb, Ricinus communis | gi|255577568 | gi|76867096 | 1 | 1.7 | – | 0.003 | 604/11 | 60.0/5.9 | 63.0/5.9 |
| 44 | Chaperonin T-complex protein 1 subunit epsilon, Zea mays | gi|226506102 | 1.5 | 2.5 | 0.05 | 0.002 | 204/5 | 59.6/5.7 | 59.0/5.6 | |
| Protein degradation | ||||||||||
| 45 | Chaperonin 21 precursorb, Solanum lycopersicum | gi|7331143 | gi|52839170 | –1.9 | 1 | 0.04 | 1 | 212/4 | 26.5/6.8 | 25.2/5.4 |
| 46 | Ubiquitin, Nicotiana benthamiana | gi|213868277 | 1 | 2.7 | – | 0.03 | 222/4 | 7.5/5.7 | 7.5/6.1 | |
| 47 | 20S proteasome subunit beta-6b, Petunia hybrida | gi|17380185 | gi|92037305 | 1 | 1.7 | – | 0.003 | 367/5 | 24.6/6.3 | 26.7/5.7 |
| 48 | 20S proteasome alpha 6 subunit, Nicotiana benthamiana | gi|22947842 | <0.01 | <0.01 | – | – | 153/4 | 29.8/5.0 | 33.0/5.2 | |
| 49 | 26S proteasome non-ATPase regulatory subunit, putative, Ricinus communis | gi|255538376 | 1 | <0.01 | – | – | 97/2 | 27.2/6.1 | 32.0/6.5 | |
| 50 | 26S protease regulatory subunit, putativeb, Ricinus communis | gi|255570523 | gi|94328305 | 1.6 | –1.7 | 0.05 | 0.03 | 610/11 | 49.5/5.9 | 58.0/6.2 |
| 51 | Putative alpha7 proteasome subunit, Nicotiana tabacum | gi|14594925 | –1.6 | 1 | 0.03 | – | 116/4 | 27.2/6.1 | 27.0/6.1 | |
| 52 | Cysteine proteinase aleuran typeb, Nicotiana benthamiana | gi|71482942 | gi|76866797 | –4.7 | <0.01 | 0.008 | – | 158/3 | 39.2/6.9 | 29.5/6.2 |
| 53 | ATP-dependent Clp protease ATP–binding subunit clpA homologue CD4B, chloroplastic, Solanum lycopersicum | gi|399213 | –2.4 | –2 | 0.04 | 0.02 | 692/15 | 102.5/5.7 | 96.0/5.5 | |
| 54 | Oligopeptidase Ab, Ricinus communis | gi|255572579 | gi|190876913 | –8 | 1 | 0.03 | – | 247/4 | 88.0/5.2 | 76.0/5.6 |
| 55 | Oligopeptidase A, putativeb, Ricinus communis | gi|255572579 | gi|190876913 | –5.6 | 1 | 0.03 | – | 87/2 | 88.0/5.2 | 78. /5.5 |
| 56 | Oligopeptidase A, putative, Ricinus communis | gi|255572579 | gi|76868752 | 1.9 | –1.8 | 0.05 | 0.01 | 524/11 | 88.0/5.2 | 77.0/5.4 |
| 57 | Mitochondrial processing peptidaseb, Solanum tuberosum | gi|587566 | gi|190874528 | 1 | –1.8 | – | 0.003 | 459/8 | 59.9/6.2 | 60.0/6.0 |
| 58 | Mitochondrial processing peptidase, Solanum tuberosum | gi|587564 | –2.6 | 1 | 0.04 | – | 281/8 | 59.4/6.2 | 60.0/5.6 | |
| 59 | Cytochrome c reductase-processing, peptidase subunit IIb, Solanum tuberosum | gi|410634 | gi|224697460 | 1 | –2 | – | 0.013 | 59.3/6.2 | 59.0/5.7 | |
| Signal transduction/regulation | ||||||||||
| 60 | BTF3, Nicotiana benthamiana | gi|90823167 | <0.01 | <0.01 | – | – | 100/3 | 17.3/6.3 | 19.0/6.5 | |
| 61 | BTF3, Nicotiana benthamiana | gi|90823167 | 2.4 | 2 | 0.05 | 0.0009 | 195/4 | 17.3/6.3 | 19.4/6.6 | |
| 62 | Nucleic acid-binding protein, putativeb, Ricinus communis | gi|255558037 | gi|190847542 | <0.01 | –1.7 | – | 0.03 | 454/7 | 28.6/4.9 | 32.0/5.4 |
| 63 | DNA-binding protein GBP16, Oryza sativa Japonica Group | gi|2511541 | <0.01 | <0.01 | – | – | 74/2 | 43.4/6.5 | 26.0/6.1 | |
| 64 | EBP1, Solanum tuberosum | gi|116292768 | gi|94324881 | 1 | –2.3 | – | 0.02 | 288/7 | 42.8/6.3 | 49.0/6.4 |
| 65 | SGT1-like protein, Nicotiana tabacum | gi|29468339 | <0.1 | <0.1 | – | – | 138/3 | 41.4/5.2 | 33.0/5.4 | |
| 66 | SGT1-like protein, Nicotiana tabacum | gi|29468339 | 2.4 | 1.6 | 0.015 | 0.03 | 167/3 | 41.4/5.2 | 46.0/5.1 | |
| 67 | Hypothetical protein RNA bindingb, Vitis vinifera | gi|296081884 | gi|190733368 | –1.7 | 1 | 0.02 | – | 253/5 | 43.7/5.8 | 62.5/5.4 |
| 68 | Hypothetical protein DNA helicase, putativeb, Vitis vinifera | gi|147858961 | gi|190806396 | 2.5 | 1 | 0.002 | – | 219/5 | 51.4/5.3 | 53.0/5.6 |
| 69 | DNA helicase, putative, Ricinus communis | gi|255565715 | 2.1 | 1 | 0.034 | – | 476/8 | 50.2/5.8 | 54.0/5.8 | |
| 70 | Adenosine kinase isoform 1T, Nicotiana tabacum | gi|51949796 | 1.5 | 1.6 | 0.05 | 0.02 | 125/2 | 37.8/5.1 | 39.0/4.8 | |
| 71 | Adenosine kinase isoform 1T, Nicotiana tabacum | gi|51949796 | 1 | –1.5 | – | 0.007 | 608/11 | 37.8/5.1 | 40.0/4.9 | |
| Carbohydrate metabolism | ||||||||||
| 72 | GAPDH, Nicotiana tabacum | gi|120676 | –1.8 | –2.2 | 0.05 | 0.0005 | 135/4 | 35.5/6.1 | 36.0/6.8 | |
| 73 | GAPDH, Nicotiana tabacum | gi|120676 | 4.8 | 6.4 | 0.001 | 0.002 | 750/17 | 35.5/6.1 | 36.5/6.6 | |
| 74 | GAPDH, Nicotiana tabacum | gi|120676 | >100 | 1 | – | – | 453/10 | 35.5/6.1 | 36.5/6.3 | |
| 75 | Enolase, Nicotiana tabacum | gi|119354 | 2.2 | 2 | 0.008 | 0.02 | 223/4 | 48.0/5.6 | 50.0/5.9 | |
| 76 | Phosphoenolpyruvate carboxylase, Glycine max | gi|399182 | 1 | –2.7 | – | 0.0001 | 74/2 | 110.6/5.7 | 110/5.8 | |
| 77 | Phosphoglycerate kinase, chloroplastic pecursor, Nicotiana tabacum | gi|2499497 | 1 | –1.7 | – | 0.04 | 563/10 | 50.3/8.5 | 40.0/5.8 | |
| 78 | Alcohol dehydrogenase, Nicotiana tabacum | gi|551257 | –1.8 | –2.7 | 0.007 | 0.01 | 661/14 | 41.9/6.6 | 42.0/6.4 | |
| 79 | Alcohol dehydrogenase, Nicotiana tabacum | gi|551257 | 2.1 | 6 | 0.01 | 0.05 | 442/10 | 41.9/6.6 | 42.0/6.1 | |
| 80 | Alcohol dehydrogenase class IIIb, Solanum lycopersicum | gi|283825505 | gi|285193921 | 2.2 | 8 | 0.05 | 0.001 | 100/4 | 40.7/6.3 | 43.0/6.9 |
| 81 | Cytosolic aconitase, Nicotiana tabacum | gi|11066033 | 1 | 5 | – | 0.03 | 134/3 | 98.7/5.8 | 108/6.1 | |
| 82 | Putative aconitaseb, Capsicum chinense | gi|171854675 | gi|92028044 | 1 | <0.01 | – | 0.041 | 131/3 | 108/7.0 | 48.0/6.0 |
| 83 | Pyruvate decarboxylase isozyme 1, Nicotiana tabacum | gi|1706327 | 2.3 | 1 | 0.003 | – | 407/7 | 45.7/6.6 | 60.0/5.7 | |
| 84 | Succinate dehydrogenase, putativeb, Ricinus communis | gi|255579273 | gi|190782074 | <0.1 | <0.1 | – | – | 181/3 | 68.5/6.2 | 73.0/5.2 |
| 85 | NADH-ubiquinone oxidoreductase, putative, Ricinus communis | gi|255582280 | 1 | –1.7 | – | 0.001 | 360/4 | 80.8/5.9 | 85.0/6.1 | |
| 86 | NAD-dependent isocitrate dehydrogenase, Nicotiana tabacum | gi|3790188 | 1 | 7 | – | 0.03 | 334/7 | 40.6/7.2 | 40.6/ 6.2 | |
| 87 | ADH-like UDP-glucose dehydrogenase, Nicotiana tabacum | gi|48093455 | –1.9 | –2.2 | 0.03 | 0.05 | 796/17 | 42.0/6.2 | 42.0/6.7 | |
| 88 | ADH-like UDP-glucose dehydrogenase, Nicotiana tabacum | gi|48093455 | 3.7 | 2.2 | 0.04 | 0.03 | 819/17 | 42.0/6.2 | 42.0/6.5 | |
| 89 | UTP-glucose-1-phosphate uridylyltransferase, Solanum tuberosum | gi|17402533 | 2:00 | 1 | 0.03 | – | 494/9 | 52.0/5.4 | 54.0/5.85 | |
| 90 | UDP-glucose:protein transglucosylase-likeb, Solanum tuberosum | gi|77416931 | gi|123218663 | 1.7 | 1 | 0.001 | – | 362/8 | 41.1/5.6 | 38.0/6.1 |
| Amino acid metabolism | ||||||||||
| 91 | Diaminopimelate epimerase, putativeb, Ricinus communis | gi|255584553 | gi|83422108 | 1 | –2 | – | 0.0005 | 377/9 | 40.1/6.0 | 35.0/5.2 |
| 92 | Aspartate semialdehyde dehydrogenase family proteinvb, Arabidopsis thaliana | gi|15223910 | gi|39863720 | 6.1 | 9 | 0.001 | 0.008 | 266/6 | 40.7/6.5 | 39.0/5.5 |
| 93 | Aspartate semialdehyde dehydrogenase, putativeb, Nicotiana sylvestris | gi|255584961 | gi|190769575 | –1.8 | –1.7 | 0.04 | 0.03 | 306/7 | 41.2/8.2 | 37.2/5.6 |
| 94 | Putative 3-isopropylmalate dehydrogenase large subunit, Capsicum annuum | gi|193290700 | <0.01 | 1 | – | – | 170/4 | 43.7/5.9 | 40.0/5.3 | |
| 95 | Isovaleryl-CoA dehydrogenase 2, mitochondrialb, Solanum tuberosum | gi|25453061 | gi|83422448 | 1.8 | 1 | 0.01 | – | 166/63 | 43.9/6.1 | 40.0/6.1 |
| 96 | Glutamine synthetase, Nicotiana plumbaginifolia | gi|121373 | 1.8 | 2.3 | 0.05 | 0.04 | 117/4 | 39.0/5.5 | 38.0/5.2 | |
| 97 | D−3−phosphoglyceratedehydrogenase,putative, Ricinus communis | gi|255555301 | gi|39856030 | –5.6 | 1 | 0.007 | – | 575/9 | 63.0/7.7 | 62.7/6.3 |
| 98 | EDA9 (embryo sac development arrest 9), ATP binding, Arabidopsis thaliana | gi|15235282 | >100 | 1 | – | – | 97/2 | 63.5/6.2 | 64.0/6.3 | |
| 99 | Chain A, structure of threonine synthase, Arabidopsis thaliana | gi|15825882 | <0.01 | 1 | – | – | 92/2 | 53.5/5.6 | 54.7/6.6 | |
| 100 | S-Adenosylmethionine synthase 2, Solanum lycopersicum | gi|1170938 | <0.01 | 1 | – | – | 112/3 | 43.0/5.4 | 45.0/5.9 | |
| Energy pathway | ||||||||||
| 101 | ATP synthase subunit delta', mitochondrialc, Ipomoea batatas | gi|2493046 | –1.8 | 2.2 | 0.007 | 0.01 | 55/1 | 21.3/5.9 | 20.0/4.7 | |
| 102 | Mitochondrial ATPase beta subunit, Nicotiana sylvestris | gi|11228579 | <0.01 | <0.01 | – | – | 622/15 | 59.6/5.2 | 48.0/5.1 | |
| 103 | Vacuolar H+-ATPase B subunit, Nicotiana tabacum | gi|6715512 | 1.6 | 1.5 | 0.004 | 0.04 | 351/8 | 53.8/5.1 | 55.0/5.1 | |
| 104 | Electron carrier/oxidoreductaseb, Arabidopsis thaliana | gi|15232542 | gi|92032774 | –1.8 | –2.3 | 0.05 | 0.001 | 218/4 | 37.2/5.7 | 36.0/6.2 |
| Cellular metabolism | ||||||||||
| 105 | Putative carbamoyl phosphate synthase small subunit, Nicotiana tabacum | gi|21535793 | –2.7 | 1 | 0.04 | – | 100/4 | 47.7/6.0 | 42.0/5.9 | |
| 106 | N-Carbamoylputrescine amidaseb, Solanum tuberosum | gi|118572820 | gi|92015448 | 1.5 | 2.2 | 0.05 | 0.007 | 220/6 | 33.4/5.9 | 33.0/6.4 |
| 107 | N-Carbamoylputrescine amidaseb, Solanum tuberosum | gi|118572820 | gi|92015448 | –5.4 | <0.01 | 0.03 | – | 199/5 | 33.4/5.9 | 33.4/6.6 |
| 108 | Spermidine synthase, Nicotiana sylvestris | gi|6094336 | 1.7 | 1 | 0.01 | – | 460/8 | 34.5/5.2 | 31.4/5.3 | |
| 109 | Spermidine synthase, Nicotiana sylvestris | gi|6094336 | 2.4 | 1 | 0.02 | – | 347/8 | 34.5/5.2 | 31.8/4.9 | |
| 110 | Spermidine synthase, Nicotiana sylvestris | gi|6094336 | <0.01 | 1 | – | – | 198/4 | 34.5/5.2 | 32.0/5.1 | |
| 111 | Putative pyridoxine biosynthesis protein isoform A, Nicotiana tabacum | gi|46399269 | 2.1 | 1.7 | 0.03 | 0.0002 | 709/12 | 33.0/5.9 | 31.0/5.8 | |
| 112 | Putative pyridoxine biosynthesis protein isoform A, Nicotiana tabacum | gi|46399269 | –1.9 | 1 | – | 347/5 | 33.0/5.9 | 31.0/6.1 | ||
| 113 | 5'-Aminoimidazole ribonucleotide synthetaseb, Solanum tuberosum | gi|37983566 | gi|190730691 | –1.9 | –1.8 | 0.03 | 0.004 | 109/2 | 42.9/5.2 | 33.0/5.3 |
| 114 | Putative 4-methyl-5 (β-hydroxyethyl)-thiazol monophosphate biosynthesis enzymec, Capsicum chinense | gi|171854671 | –1.5 | <0.01 | 0.04 | – | 77/1 | 41.7/5.4 | 40.0/5.9 | |
| 115 | Putative 4-methyl-5 (β-hydroxyethyl)-thiazol monophosphate biosynthesis enzymeb, Capsicum chinense | gi|171854671 | gi|190775218 | <0.01 | <0.01 | – | – | 225/5 | 41.7/5.4 | 37.0/5.6 |
| 116 | Putative 4-methyl-5 (β-hydroxyethyl)-thiazol monophosphate biosynthesis enzymeb, Capsicum chinense | gi|171854671 | gi|47004010 | >100 | >100 | – | – | 232/3 | 41.7/5.4 | 40.0/5.5 |
| 117 | Betaine-aldehyde dehydrogenaseb, Nicotiana tabacum | gi|92037527 | 1 | –1.6 | – | 0.006 | 187/3 | 59.7/5.4 | 57.3/5.6 | |
| 118 | Putative cinnamyl alcohol dehydrogenaseb, Nicotiana tabacum | gi|156763848 | gi|190749158 | 1 | 1.8 | – | 0.02 | 82/2 | 38.9/6.6 | 39.8/6.5 |
| 119 | NAD-dependent epimerase/dehydratase, putativeb, Ricinus communis | gi|255537241 | gi|190751083 | >100 | >100 | – | – | 261/5 | 34.0/6.2 | 31.0/6.6 |
| 120 | NAD dependent epimerase/dehydratase, putativeb, Ricinus communis | gi|255537241 | gi|92028282 | 3.5 | 2.9 | 0.007 | 0.004 | 440/10 | 34.0/6.2 | 30.0/6.5 |
| 121 | NAD-dependent epimerase/dehydratase, putativeb, Ricinus communis | gi|255537241 | gi|92028282 | –8.1 | –7 | 0.04 | 0.004 | 476/10 | 34.0/6.2 | 30.0/6.8 |
| 122 | Type 2 proly 4–hydroxylaseb, Nicotiana tabacum | gi|215490181 | gi|190760427 | >100 | >100 | – | – | 219/3 | 32.7/6.3 | 29.0/6.2 |
| 123 | Gibberellin 20 oxidase, putative, Ricinus communis | gi|255556243 | –2.5 | <0.01 | 0.02 | – | 126/2 | 42.0/5.7 | 39.0/5.3 | |
| 124 | 1-Aminocyclopropane-1-carboxylate oxidaseb, Solanum lycopersicum | gi|50830975 | gi|39853392 | 1.7 | –1.6 | 0.001 | 0.03 | 114/2 | 34.4/6.1 | 32.0/6.2 |
| 125 | Acireductone dioxygenaseb, Solanum tuberosum | gi|158325159 | gi|76867763 | <0.01 | <0.01 | – | – | 237/4 | 23.3/4.8 | 24.0/4.8 |
| 126 | Acireductone dioxygenaseb, Solanum tuberosum | gi|158325159 | gi|76866491 | 1 | <0.01 | – | – | 149/4 | 23.3/4.8 | 24.6/4.9 |
| 127 | Rubisco subunit binding-protein alpha subunitb, Ricinus communis | gi|255587664 | gi|190794529 | –1.5 | –1.5 | 0.05 | 0.04 | 895/9 | 53.2/5.2 | 61.0/4.8 |
| 128 | SAL1; phosphatidylinositol phosphataseb, Arabidopsis thaliana | gi|145359623 | gi|190143393 | 1.5 | 1.6 | 0.05 | 0.02 | 353/6 | 43.4/6.0 | 40.0/5.3 |
| 129 | Patatin homologue, Nicotiana tabacum | gi|1546817 | –1.9 | –1.8 | 0.05 | 0.02 | 363/6 | 42.5/5.1 | 41.0/5.2 | |
| 130 | Acyl-[acyl-carrier-protein] desaturase, chloroplasticb, Solanum commersoni | gi|94730426 | gi|92012186 | <0.01 | –1.9 | – | 0.03 | 376/8 | 44.8/6.3 | 38.6/5.9 |
| 131 | DH putative beta-hydroxyacyl-ACP dehydrataseb, Capsicum annuum | gi|193290688 | gi|52834057 | >100 | >100 | – | – | 146/2 | 23.9/9.4 | 23.6/6.2 |
| 132 | Putative pyruvate dehydrogenase E1 alpha subunit, Capsicum annuum | gi|193290722 | <0.01 | <0.01 | – | – | 159/5 | 48.0/6.3 | 40.0/6.6 | |
| 133 | Glutamate–cysteine ligase, chloroplastic, Nicotiana tabacum | gi|122194121 | –2.5 | –1.9 | 0.05 | 0.05 | 304/6 | 59.4/6.2 | 46.3/5.7 | |
| 134 | L-Galactono-gamma-lactone dehydrogenase, Nicotiana tabacum | gi|6519872 | <0.01 | –2 | – | 0.04 | 350/9 | 67.1/7.7 | 54.0/6.6 | |
| 135 | Putative ketol-acid reductoisomerase, Capsicum annuum | gi|193290660 | –2.6 | 1 | 0.04 | – | 281/8 | 63.7/6.5 | 60.0/5.7 | |
| 136 | Prolyl endopeptidase, putativeb, Ricinus communis | gi|255539116 | gi|190876295 | –2.3 | –1.9 | 0.04 | 0.008 | 301/5 | 80.0/5.3 | 95.0/5.2 |
| 137 | Short chain dehydrogenaseb, Solanum tuberosum | gi|77403673 | gi|254640456 | <0.01 | 1 | – | – | 139/3 | 27.2/6.2 | 26.8/6.1 |
| Cell structure | ||||||||||
| 138 | Actin isoform Bb, Mimosa pudica | gi|6683504 | gi|39877476 | <0.01 | <0.01 | – | – | 153/4 | 41.7/5.3 | 36.0/5.7 |
| 139 | Actin, Nicotiana tabacum | gi|197322805 | <0.01 | <0.01 | – | – | 72/1 | 41.7/5.3 | 35.0/5.7 | |
| 140 | Actin-binding protein ABP29b, Lilium longiflorum | gi|117553552 | gi|190829672 | –2.7 | –5.5 | 352/6 | 29.4/6.0 | 38.0/5.5 | ||
| 141 | Actin, Nicotiana tabacum | gi|50058115 | gi|39805809 | 2.5 | 2.6 | 0.05 | 0.03 | 147/5 | 41.8/5.3 | 43.0/5.2 |
| 142 | alpha-Tubulin, Nicotiana tabacum | gi|11967906 | 1 | –2 | 1 | 0.002 | 124/2 | 50.4/4.9 | 49.0/6.5 | |
| 143 | Tubulin beta-2 chainb, Anemia phyllitidis | gi|297826947 | gi| 56691710 | <0.01 | 1 | – | – | 114/4 | 46.8/4.9 | 55/6.8 |
| 144 | Predicted proteinb,. Populus trichocarpa | gi|224125262 | gi|39875797 | 1.5 | 1.5 | 0.04 | 0.02 | 322/7 | 26.0/5.1 | 30.0/5.7 |
| 145 | Villin 3 fragmentc, Arabidopsis thaliana | gi|6735320 | <0.01 | 1 | – | – | 63/1 | 64.8/5.5 | 38.0/5.4 | |
| 146 | Hypothetical protein stomatin-like proteinb, Vitis vinifera | gi|225442194 | gi|92027149 | 1.7 | 1 | 0.03 | – | 553/8 | 45.6/9.0 | 39.0/6.8 |
| 147 | Hypothetical protein isoform 2b, Vitis vinifera | gi|225454579 | gi|190805443 | 3.2 | 1 | 0.0003 | – | 230/5 | 17.0/4.7 | 21.0/4.6 |
| 148 | Hypothetical protein coatomer delta subunitb, Vitis vinifera | gi|270239956 | gi|190738155 | <0.01 | <0.01 | – | – | 226/5 | 61.5/5.6 | 30.0/4.9 |
| 149 | Stem-specific protein TSJT1, putativeb, Ricinus communis | gi|255552037 | gi|92026938 | 1 | –2.4 | – | 0.002 | 380/7 | 27.0/6.0 | 27.8/6.3 |
| 150 | Stem-specific protein TSJT1, putativeb, Ricinus communis | gi|255552037 | gi|92026938 | 3.8 | 1 | 0.02 | – | 448/10 | 27.0/6.0 | 28.0/6.6 |
A value >100 (<0.01) means that the protein spot is detected (is not present) under the condition reporting this value.
a
Tests of statistical significance of changes in spot volumes utilized a Student’s t-test, and levels of P ≤ 0.05 were considered significant.
b
Protein found in the EST NCBI database. The name, the accession number, the molecular weight, and the isoelectric point were annotated by BLAST search
c
Protein confirmed by de novo analysis.