Table 2.

Profiles of free amino acids in developing seeds at the 100 mg stage of genetically related soybean cultivars differing in protein concentration

Cultivara	Aspartate	Glutamate	Asparagine	Serine	Gluatmine	Glycine	Histidine	Citrulline
Maple Arrow	0.22±0.02	0.78±0.07	2.2±0.2	0.20±0.01	1.0±0.1	0.047±0.005	0.55±0.07	0.098±0.012
AC Hercule	0.40±0.16	1.54±0.58	4.9±1.8	0.46±0.21	3.7±1.5	0.102±0.045	1.10±0.56	0.253±0.112
AC Proteus	1.07±0.24	4.03±1.04	15.8±3.2	1.19±0.21	9.2±1.3	0.276±0.057	3.26±0.64	0.772±0.177
LSD	0.29	1.24	2.7	0.29	2.0	0.087	0.86	0.215
ANOVA P-value	0.002	0.005	0.001	0.0001	0.0001	0.0003	0.0001	0.0001

Cultivar	Arginine	GABA	Threonine	Alanine	Proline	ANBA	Tyrosine	Valine
Maple Arrow	0.36±0.04	0.16±0.04	0.055±0.004	0.42±0.03	0.034±0.002	0.003±0.005	0.030±0.002	0.069±0.005
AC Hercule	0.95±0.37	0.31±0.15	0.111±0.050	0.91±0.46	0.076±0.036	0.024±0.018	0.091±0.041	0.166±0.072
AC Proteus	2.95±0.81	0.72±0.15	0.285±0.061	2.09±0.84	0.189±0.033	0.056±0.011	0.204±0.028	0.447±0.062
LSD	0.93	0.215	0.079	0.99	0.048	0.020	0.048	0.092
ANOVA P-value	0.0003	0.0005	0.0002	0.009	0.0001	0.0006	0.0001	0.0001

Cultivar	Methionine	Isoleucine	Leucine	Phenylalanine	Ornithine	Lysine	Total free amino acids
Maple Arrow	0.078±0.010	0.043±0.003	0.043±0.004	0.033±0.004	0.008±0.003	0.023±0.004	6.5±0.5
AC Hercule	0.211±0.126	0.107±0.044	0.132±0.051	0.074±0.034	0.013±0.007	0.047±0.025	15.7±6.5
AC Proteus	0.597±0.145	0.265±0.027	0.365±0.049	0.226±0.041	0.062±0.010	0.144±0.038	44.3±7.6
LSD	0.192	0.048	0.069	0.053	0.013	0.047	10.0
ANOVA P-value	0.0004	0.0001	0.0001	0.0001	0.0001	0.0005	0.0001

Values are expressed in nmol mg⁻¹ seed weight. Mean ±SD; n=4.

^aProtein concentration was equal to 40.9% for Maple Arrow, 45.7% for AC Hercule, and 48.0% for AC Proteus.

ANBA, amino-N-butyric acid; GABA, γ-aminobutyric acid.