Fig. 1.

Physcomitrella patens genes encoding the group A-type Dof domain. (A) Alignment of the deduced amino acid sequences of PpDof1–PpDof6. Amino acid sequence motifs similar to small structural motifs that were previously found in the N-terminal regions of angiosperm group-A Dof factors are indicated by boxes I and II. The Dof domains are indicated by box III. Amino acid sequences that are conserved in PpDof1–PpDof4 are indicated by box IV. Two amino acid sequence motifs similar to small structural motifs that were found in the C-terminal regions of Arabidopsis CDF1–CDF3 are indicated by boxes V and VI. (B) Schematic representation of the intron–exon structures of PpDof1–PpDo6 genes. Exons and introns are indicated by boxes and bars, respectively. The 5′- and 3′-untranslated regions, coding regions, and the sequence encoding the Dof domain are indicated by white, grey, and black boxes, respectively. Translational start codons (ATG) and stop codons (TGA or TAG) are also shown. (C) Expression analysis by RT-PCR. Reverse transcription reactions were performed in the presence (+) or absence (–) of reverse transcriptase using RNA prepared from gametophores (G) and protonema (P). Transcripts of the α-tubulin gene (PpTUA2) (accession number AB096719) were detected as a control.