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. 2012 Feb 15;63(8):3185–3197. doi: 10.1093/jxb/ers042

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Nutrient condition-dependent phenotype of the disruptant lines. (A) Areas of protonemal colonies that were initially grown on regeneration medium for 4 weeks and then on BCD medium supplemented with ammonium and glucose (BCD+A+G), BCD medium supplemented with ammonium (BCD+A), or BCD medium for the indicated periods after protoplast formation. Values are the means ±SD of three replicates. (B) Images showing the branching of protonemal filaments of wild-type (WT), ppdof1 or ppdof2 single disruptant lines, and the ppdof1 ppdof2 double disruptant line. Scale bars=200 μm. (C) The numbers of branches within six cells from the filament top. These numbers were measured using protonemal colonies that were initially grown on regeneration medium plates for 4 weeks and then on BCD medium supplemented with ammonium and glucose (BCD+A+G), BCD medium supplemented with ammonium (BCD+A), or BCD medium for 3 weeks. Values are the means ±SD of 15 replicates. Asterisks indicate a statistically significant difference (P < 0.05), compared with the values obtained for wild-type P. patens.