Fig. 2.

Southern and northern analysis of rice lines. (a) A 20 μg aliquot of genomic DNA was digested by EcoRV, fractioned on a 0.8% agarose gel, transferred onto a nylon membrane, and hybridized with a fragment of hpt. Size markers are shown on the left in kilobases. (b) A 10 μg aliquot of total RNA was fractioned on a 1.2% agarose–formaldehyde gel, transferred onto a nylon membrane, and hybridized with a fragment encoding GmFAD3-1 or OsFAD3. NT, Kitaake, non-transformants; GG, transformant with the GmFAD3-1 gene directed by the GluC promoter; GO, transformants with the OsFAD3 gene directed by the GluC promoter.