Fig. 3.

In situ western hybridization and western blot analysis of FAD3 in transgenic rice seeds. (a) In situ western hybridization of FAD3 transgenic rice seeds. Scale bar=0.1 mm. (b) Western blot analysis of FAD3 in transgenic rice seeds. Total proteins extracted from each transgenic and non-transgenic seed were fractioned by SDS–PAGE and immunoblotted with the GmFAD3-1 or OsFAD3 rabbit polyclonal antibodies. The numbers above each lane indicate the relative multiples of FAD3 levels. NT, GG, and GO are as described in Fig. 2.