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. 2012 Feb 29;63(8):3279–3287. doi: 10.1093/jxb/ers051

Table 1.

Primers used for cloning of FAD genes

Primer name Primer sequence (with the restriction site underlined)
OsFAD3F 5′-AACCCGGGATGGCGGCGTCGGCGACCCAG-3′ (SmaI)
OsFAD3R 5′-AAGAGCTCTCACTTGTGCTTAGCATCTTC-3′ (SacI)
OsFAD7F 5′-AAGGATCCATGGCACGGCTCGTCCTCTCCG-3′(BamHI)
OsFAD7R 5′-AAGAGCTCTCAATCCGAGCTTTGTGCAGAA-3′ (SacI)
OsFAD8F 5′-AAGGATCCATGGCCCGGCTGCTGCTCTCCG-3′(BamHI)
OsFAD8R 5′-AAGAGCTCTTAGTTAGCAGGGTCGGTCTGG-3′ (SacI)
GmFAD3-1F 5′-AACCCGGGATGGTTAAAGACACAAAG-3′ (SmaI)
GmFAD3-1R 5′-AAGAGCTCTCAGTCTCGTTGCGAGTGGA-3′ (SacI)
GmFAD3-2F 5′-AAGCGGCCGCCATGGTTAAAGACACAAAGCCTT-3′ (NotI)
GmFAD3-2R 5′-AAGCGGCCGCTCAGTCTCGTTGCGAGTGGAGG-3′ (NotI)
GmFAD3-3F 5′-AAGCGGCCGCCATGGTTCAAGCACAGCCTCTAC-3′ (NotI)
GmFAD3-3R 5′-AAGCGGCCGCTTAGTTGGACTGGGTCCAAGAA-3′ (NotI)