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. Author manuscript; available in PMC: 2012 May 14.
Published in final edited form as: Mol Cell Neurosci. 2010 Jan 18;43(4):353–362. doi: 10.1016/j.mcn.2010.01.002

Figure 4.

Figure 4

Lrrc3b::tauYFP expression in genetically modified mice. (A) Schematic diagram of the tau-YFP reporter knock-in genomic structure. The reporter and neo selectable marker replace the single exon that contains the complete Lrrc3b coding region. (B) Membrane-enriched OE extract from wild type mice (lane 2) contains a protein band that comigrates with HEK293-expressed Lrrc3b protein (lane 1) and is absent in Lrrc3b(ΔYFP/ΔYFP) mice (lane 3). Lrrc3b protein is visualized with affinity purified Lrrc3b antisera. A cross-reacting band of higher molecular weight does not change in abundance. (C) Visualization of intrinsic YFP fluorescence confirms the variation in intensity among ORN cell bodies in Lrrc3b(ΔYFP/+) adult OE. Fewer intensely labeled cells are observed in Lrrc3b(ΔYFP/+) olfactory epithelium at PD10 (D) and PD20 (E). scale bar = 25 microns