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. Author manuscript; available in PMC: 2012 May 14.
Published in final edited form as: Virology. 2009 Dec 11;397(2):389–398. doi: 10.1016/j.virol.2009.11.011

Fig. 5.

Fig. 5

Use of immunofluorescence to confirm reduced 3-OS HS expression. HeLa cells were incubated with the antibody HS4C3 for 1 h at 4 °C. Cells were then fixed for 20 min, and incubated with FITC conjugated anti-mouse IgG to label 3-OS HS surface expression. 3-OS HS surface expression was compared in HeLa cells that were mock treated (left panels) or transfected with scrambled siRNA (middle panels) or 2-OST siRNA (right panels). Imaging was performed using confocal microscopy at a 60x oil objective.