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. Author manuscript; available in PMC: 2012 May 14.
Published in final edited form as: Photochem Photobiol. 2009 Apr 6;85(5):1189–1200. doi: 10.1111/j.1751-1097.2009.00558.x

Figure 7.

Figure 7

Kinetics of apoptosis-related events post-PDT. Cells were treated with Pc 4- or Pc 181-PDT at the concentrations to yield 90% lethality for each. Cells were harvested by scraping at the indicated time postirradiation (200 mJ cm−2). Cell lysates were subjected to electrophoresis on a 12% polyacrylamide gel to monitor pro- and active caspase-3 and Bid or an 8% polyacrylamide gel to determine PARP cleavage with appropriate antibodies. Actin was used as a loading control. As a positive control, some cells were treated with 1 μM staurosporine (STS) for 6 h. Results were similar in two additional independent experiments. (A) Caspase activation. (B) Cleavage of Bid to generate truncated Bid (tBid). For Pc 181, both LD90 and LD99 doses were studied. C, untreated control. (C) PARP cleavage post-PDT.