Table 1.
Primary Antibodies
| Antibody (Diaminobenzidine-Based Staining) | Host | Clone | Dilution | Blocking Serum 3% |
|---|---|---|---|---|
| Anti-actin | Mouse | ACTN05 (C4) | 1:100 | Goat |
| Anti–myosin II | Rabbit | Polyclonal | 1:2000 | Goat |
| Anti-cofilin | Rabbit | Polyclonal | 1:1000 | Goat |
| Anti-profilin | Rabbit | Polyclonal | 1:50 | Goat |
| Anti-SNAP-23 | Rabbit | Polyclonal | 1:100 | Goat |
| Anti-syntaxin-2 | Goat | Polyclonal | 1:100 | Rabbit |
| Anti-syntaxin-4 | Rabbit | Polyclonal | 1:300 | Goat |
| Anti-VAMP-2 | Rabbit | Polyclonal | 1:100 | Goat |
| Anti-septin-2 | Goat | Polyclonal | 1:80 | Rabbit |
| Anti-body (Immunofluorescence) | Host | Clone | Dilution | Blocking Serum 2% |
| Anti-septin-2 | Rabbit | Polyclonal | 1:250 | BSA |
| Anti-syntaxin-2 | Goat | Polyclonal | 1:50 | BSA |
| Anti-lactoferrin | Mouse | Monoclonal [2B8] | 1:1000 | BSA |
| Anti-α-amylase | Mouse | Monoclonal | 1:250 | BSA |