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. 2011 May 25;31(21):7817–7830. doi: 10.1523/JNEUROSCI.6412-10.2011

Figure 8.

Figure 8.

Recovery of lysosomal proteolysis restores transport of GFP-Rab7-positive late endosomes and autolysosomes. A, Active cathepsin B (Magic Red) is severely reduced by leupeptin treatment (20 μm, 24 h) but restored after recovery for 3 d in normal medium. B, Quantification of Magic Red cathepsin B loading in A. Values represent mean intensity. C, Quantification of GFP-Rab7 movements after leupeptin (n = 55 vesicles), or 24 h recovery after leupeptin (leupeptin recovery; n = 119) compared with untreated controls (n = 101 vesicles) and recovery controls (normal medium replacement without leupeptin treatment; n = 119 vesicles). D, Leupeptin recovery restores the percentage of moving vesicles and the retrograde transport of GFP-Rab7 vesicles (C, D). Values represents means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.