Table 1.

Time-lapse summary of GFP-LC3, YFP-LAMP, and LysoTracker Red vesicles after rapamycin treatment

	GFP-LC3		YFP-LAMP		LysoTracker Red
	Control	RM	Control	RM	Control	RM
Total vesicles	107	64	82	84	62	77
Total movements	3648	1074	1975	3139	2039	3257
Mean retrograde velocity (μm/s)	0.32 ± 0.07	0.33 ± 0.02	0.54 ± 0.05	0.47 ± 0.06	0.29 ± 0.03	0.25 ± 0.02
Net anterograde vesicles	3	2	46	48	31	42
Net retrograde vesicles	95	57	32	31	29	30
Stationary vesicles	8	5	4	5	2	5

Axonal transport of GFP-LC3, YFP-LAMP, and LysoTracker Red vesicles under normal conditions (control) or after rapamycin (RM) treatment (10 nm, 5 h). RM does not significantly alter motility, movement direction, or velocity for GFP-LC3, YFP-LAMP, or LysoTracker Red vesicles.