Table 1.
Thermal and thermodynamic parameters of FAAF modified NarI duplexes obtained from differential scanning calorimetry
| 5′-CTCTCG1G2CG3CCATCAC-3′ | ||||||||
|---|---|---|---|---|---|---|---|---|
| 3′-GAGAGC C GCGGTAGTG-5′ | ||||||||
| −ΔH (kcal/mol) | −ΔS (eu) | −ΔG37°c (kcal/mol) | Tma (°C) | ΔΔHb (kcal/mol) | ΔΔSc (eu) | ΔΔG37°cd (kcal/mol) | ΔTme (°C) | |
| Controlf | 117.5 | 319.1 | 18.6 | 73.9 | – | – | – | – |
| NarI-G1-FAAFf | 95.6 | 260.2 | 14.9 | 68.6 | 21.9 | 58.9 | 3.7 | −5.3 |
| NarI-G2-FAAFf | 98.9 | 272.1 | 14.5 | 66.0 | 18.6 | 47.0 | 4.1 | −7.9 |
| NarI-G3-FAAFf | 92.8 | 254.4 | 13.9 | 65.6 | 24.7 | 64.7 | 4.7 | −8.3 |
aTm values is the temperature at half the peak area.
bΔΔH = ΔH (modified duplex) −ΔH (control duplex).
cΔΔS = ΔS (modified duplex) −ΔS (control duplex).
dΔΔG = ΔG (modified duplex) −ΔG (control duplex).
eΔTm = Tm (modified duplex) −Tm (control duplex).
fThe average standard deviations for −ΔG, −ΔH and Tm are ±0.4, ±3.0 and ±0.4, respectively.