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. 2012 Jan 24;40(9):e64. doi: 10.1093/nar/gks028

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Inversion of Dox-inducible phenotype with a mutant TetR. (a) A revTetR variant dose-dependently represses RRL translation of synthetic FLuc mRNA as in Figure 2c, but only in the presence of Dox. (b) A revTetR variant specifically represses translation as in Figure 2d, but only in the presence of Dox. In all figures, data represent the mean ± SD of at least four experiments. A two-tailed, unpaired t-test was used to calculate the significance (α = 0.005) of the difference between induced and uninduced conditions. ^¥P = 8.1 × 10⁻⁵.