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. 2012 Jan 24;40(9):e64. doi: 10.1093/nar/gks028

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Polysome profiles of aptamer-containing mRNA indicate regulation is independent of translation initiation. (a) Polysomes were fractionated from yeast expressing both TetR and a 5-1.2-containing vYFP reporter mRNA, which were grown in the absence or presence of Dox. Polysome profiles for both the − Dox and + Dox growth conditions are shown. (b) qPCR measurements of the relative amounts of reporter mRNA within each polysome fraction, both for the − Dox and + Dox conditions. (c) qPCR measurements of relative amount of ACT1 mRNA in each polysome fraction, under − Dox and + Dox conditions. For both (b) and (c) error bars indicate the range of values for technical replicates. The data are representative of two independent, biological replicates.